If you rotate them then you'll probably end up with the same dusty look, unless you're trying to go for the flying through space look. The twinkling effect is a random stepping of opacity between two decimals (like 0.5 - 0.7) where the range's center in the brightness of the star. So using that you could set a series of random number generators tied to a specific range (of whose variables could also be randomly generated) and then post that to a series of transparent star plates you have positioned about the page. You're looking at alot of work depending on how realistic you want the effect to be, as no two stars would share the same twinkling, and if you wanted to create a sparse transparent plate of stars and stack that then the effect might suffer a bit, depending on how dense the final render was in relation to the initial plate. Otherwise you're looking at setting each star either randomly or manually, in which case it will be a bear trying to apply separate twinkling to each one.
I found this as a method of generating a random number between any two numbers. Im not very advanced at jquery so someone else might be able to help with the actual coding part, but this is the gist of it.
function randomFromInterval(from,to)
{
return Math.floor(Math.random()*(to-from+1)+from);
}
Edit: Im not sure if that code constantly generates a random number of just once on pageload, it might need to be fed into a "everyTime" loop so that it constantly changes.